Brazilian Journal of Anesthesiology
https://bjan-sba.org/article/doi/10.1590/S0034-70942010000200008
Brazilian Journal of Anesthesiology
Scientific Article

Avaliação de parâmetros antioxidantes em ratos tratados com sevoflurano

Evaluation of antioxidant parameters in eats treated with sevoflurane

Francisco J. L Bezerra; Nilton Bezerra do Vale; Brunno de Oliveira Macedo; Adriana Augusto Rezende; Maria das Graças Almeida

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Resumo

JUSTIFICATIVA E OBJETIVOS: O sevoflurano é um éter halogenado com flúor que sofre biotransformação hepática através do citocromo P450 2E1. Éteres halogenados que sofrem biotransformação pelo P450 2E1 podem produzir espécies reativas do oxigênio (ERO) e promover enfraquecimento do sistema de defesa antioxidante. O objetivo deste trabalho foi investigar a relação entre a atividade das enzimas antioxidantes eritrocitárias e o sevoflurano. MÉTODO: Os animais foram distribuídos em quatro grupos: Grupo 1 controle: apenas oxigênio a 100% (1 L.min-1 por 60 minutos durante 5 dias consecutivos); Grupo 2 - sevoflurane 4,0% em oxigênio a 100% (1 L.min-1 por 60 minutos durante 5 dias consecutivos); Grupo 3 - isoniazida (i.p.), 50 mg.kg-1 de peso corporal /dia, durante 4 dias e em seguida tratados apenas com oxigênio a 100% (1 L.min-1 por 60 minutos durante 5 dias consecutivos); Grupo 4 - isoniazida por via intraperitoneal na dose de 50 mg.kg-1 de peso corporal, diariamente durante 4 dias, seguido da administração do sevoflurane a 4,0% em oxigênio a 100% (1 L.min-1 por 60 minutos durante 5 dias). Após 12 horas da última exposição ao sevoflurane, os animais foram sacrificados e o sangue foi coletado através da veia porta para análise da atividade das enzimas antioxidantes. RESULTADOS: Aumento da atividade específica da glicose-6-fosfato desidrogenase, diminuição da atividade específica da catalase, principalmente no grupo de animais pré-tratados com isoniazida e, em seguida, tratados com sevoflurano. A glutationa peroxidase não apresentou alteração na sua atividade. CONCLUSÕES: A interação do sevoflurano com indutores enzimáticos do citocromo P450 2E1 pode propiciar a instalação do estresse oxidativo caso a exposição se torne prolongada e repetitiva.

Palavras-chave

ANESTÉSICOS, Volátil, ANIMAIS, DROGAS, Antioxidantes, METABOLISMO

Abstract

BACKGROUND AND OBJECTIVES: Sevoflurane is a halogenated fluorinated ether that undergoes hepatic biotransformation through cytochrome P4502E1. Halogenated ethers undergoing biotransformation by P4502E1 can produce reactive oxygen species (ROS), weakening the antioxidant defense mechanism. The objective of this study was to investigate the relationship between the activity of erythrocyte antioxidant enzymes and sevoflurane. METHODS: Animals were divided in four groups: Group 1 - control: 100% oxygen (1 L.min-1 for 60 min during five consecutive days); Group 2 - 4.0% sevoflurane in 100% oxygen (1 L.min-1 for 60 minutes during five consecutive days); Group 3 - isoniazid (i.p.), 50 mg.kg-1/ day for four consecutive days, followed by 100% oxygen (1 L.min-1 for 60 minutes during four consecutive days); Group 4 - intraperitoneal isoniazid, 50 mg.kg-1 daily for four days, followed by 4.0% sevoflurane in 100% oxygen (1 L.min-1 for 60 minutes during five days). Twelve hours after the last exposure to sevoflurane, animals were sacrificed and their blood was collected through the portal vein for analysis of antioxidant enzymes. RESULTS: An increase in the activity of glucose-6-phosphate dehydrogenase and a decrease in the activity of catalase were observed, especially in the group of animals pre-treated with isoniazid. Changes in the activity of glutathione peroxidase were not observed. CONCLUSIONS: The interaction between sevoflurane and cytochrome P450 2E1 with enzymatic inducers can lead to oxidative stress with prolonged and repetitive exposure.

Keywords

ANESTHETICS, Volatile, ANIMALS, DRUGS, Antioxidants, METABOLISM

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