Brazilian Journal of Anesthesiology
https://bjan-sba.org/article/doi/10.1590/S0034-70942004000100007
Brazilian Journal of Anesthesiology
Scientific Article

Correlação entre concentração liquórica e efeitos colaterais após injeção de morfina por via subaracnóidea em ratos

Correlation between CSF concentration and side effects after spinal morphine injection in rats

Neuzimar de Souza Freire Silva; Rioko Kimiko Sakata; Adriana Machado Issy

http://dx.doi.org/10.1590/S0034-70942004000100007 Braz J Anesthesiol, vol.54, n1, p.53-59, 2004
PDF Português
PDF English
Downloads: 0
Views: 985

Resumo

JUSTIFICATIVA E OBJETIVOS: A morfina por via subaracnóidea promove bom efeito analgésico, mas não é isenta de efeito colateral. O objetivo deste estudo foi verificar se há correlação entre a concentração de morfina no líquor e os efeitos colaterais após injeção de morfina por via subaracnóidea. MÉTODO: Foram estudados 28 ratos, em quatro grupos, 24 horas após a colocação de cateter subaracnóideo via cisterna magna. Os grupos G1, G2, G3 e G4 receberam respectivamente 0,1; 0,3; 0,5 e 1 µg de morfina em 10 µl de solução fisiológica a 0,9%. Foram coletadas amostras de líquor e anotados os efeitos colaterais nos momentos M15, M30, M60, M120 e M180 minutos após a injeção. RESULTADOS: Os efeitos colaterais observados foram tremor mandibular, agitação, prurido, ausência de diurese, sedação e alteração respiratória. Houve maior incidência de efeitos colaterais nas avaliações precoces, diminuindo progressivamente com o tempo. As concentrações médias de morfina no líquor no G1 variou de 72,84 a 1,13 pg; no G2, de 114,26 a 5,68 pg; no G3, de 151,18 a 13,62 pg; e no G4, de 561,37 a 18,61 pg. CONCLUSÕES: Não houve correlação entre concentração de morfina no líquor e efeitos colaterais.

Palavras-chave

ANALGÉSICOS, Opióides, ANIMAL, TÉCNICAS ANESTÉSICAS, Regional

Abstract

BACKGROUND AND OBJECTIVES: Spinal morphine promotes good pain relief, but is not free from side effects. This study aimed at verifying the correlation between CSF morphine concentration and side effects.
METHODS: This study involved 4 groups of 7 rats, which were studied 24 hours after spinal catheter insertion via cisterna magna. Groups G1, G2, G3 and G4 received respectively 0.1; 0.3; 0.5 and 1 µg morphine in 10 µl of 0.9% saline solution. CSF samples were collected and side effects were recorded at M15, M30, M60, M120 and M180 minutes after injection.
RESULTS: Side effects observed were: mandible tremors, agitation, pruritus, absence of diuresis, sedation, and respiratory changes. The incidence of side effects was higher during early evaluations and progressively decreased with time. Mean CSF morphine concentrations in G1 varied from 72.84 to 1.13 pg; in G2 from 114.26 to 5.68 pg; in G3 from 151.18 to 13.62 pg and in G4, from 561.37 to 18.61 pg.
CONCLUSIONS: There has been no correlation between CSF morphine concentration and side effects.

Keywords

ANALGESICS, Opioids: morphine, ANESTHETIC TECHNIQUES, Regional: spinal block; ANIMAL: rat

References

Snyder SH, Pert CB, Pasternak GW. The opiate receptor. Ann Intern Med. 1974;81:534-540.

Hughes J, Smith T, Morgan B. Purification and properties of enkephalin: the possible endogenous ligand for the morphine receptor. Life Sci. 1975;16:1753-1758.

Yaksh L, Rudy TA. Chronic catheterization of the spinal subarachnoid space. Physiol Behav. 1976;17:1031-1036.

Van den Hoogen RHWM, Bervoets KJK, Colpaert TFC. Respiratory effects of epidural and subcutaneous morphine, meperidine, fentanyl and sufentanil in the rat. Anesth Analg. 1988;67:1071-1078.

Dib B. Intrathecal chronic catheterization in the rat. Pharmacol Biochem Behav. 1984;20:45-48.

Nishiyama T. A rat model of chronic lumbar epidural catheterization. Can J Anaesth. 1998;45:907-912.

Matos FF, Rollema H, Taiwo Y. Relationship between analgesia and extracelular morphine in brain and spinal cord in awake rats. Brain Research. 1995;693:187-195.

Bolander H, Kourtopoulos H, Lundberg S. Morphine concentrations in serum, brain and cerebrospinal fluid in the rat after intravenous administration of a single dose. J Pharm Pharmacol. 1983;35:656-659.

5dd7f6820e8825e91713f286 rba Articles
Links & Downloads
2352-2291 (Electronic) 0104-0014 (Printed)
Braz J Anesthesiol ©2024 All rights reserved.

Braz J Anesthesiol

Share this page
Page Sections