Brazilian Journal of Anesthesiology
Brazilian Journal of Anesthesiology
Original Investigation

Neuroligins facilitate the development of bone cancer pain via regulating synaptic transmission: an experimental study

Xianqiao Xie, Yang Li, Shanchun Su, Xiaohui Li, Xueqin Xu, Yan Gao, Minjing Peng, Changbin Ke

Downloads: 0
Views: 341



The underlying mechanism of chronic pain involves the plasticity in synaptic receptors and neurotransmitters. This study aimed to investigate potential roles of Neuroligins (NLs) within the spinal dorsal horn of rats in a newly established Bone Cancer Pain (BCP) model. The objective was to explore the mechanism of neuroligin involved in the occurrence and development of bone cancer pain.


Using our rat BCP model, we assessed pain hypersensitivity over time. Quantitative real-time polymerase chain reaction and Western blot analysis were performed to investigate NL expression, and NLs were overexpressed in the rat spinal cord using lentiviral vectors. Immunofluorescence staining and whole-cell patch-clamp recordings were deployed to investigate the role of NLs in the development of BCP.


We observed reduced expression levels of NL1 and NL2, but not of NL3, within the rat spinal cord, which were found to be associated with and essential for the development of BCP in our model. Accordingly, NL1 or NL2 overexpression in the spinal cord alleviated mechanical hypersensitivity of rats. Electrophysiological experiments indicated that NL1 and NL2 are involved in BCP via regulating γ-aminobutyric acid-ergic interneuronal synapses and the activity of glutamatergic interneuronal synapses, respectively.


Our observations unravel the role of NLs in cancer-related chronic pain and further suggest that inhibitory mechanisms are central features of BCP in the spinal dorsal horn. These results provide a new perspective and basis for subsequent studies elucidating the onset and progression of BCP.


Cancer pain, GABAergic, Glutamatergic, Neuroligins, Synaptic transmission


642ed4eea953954f7e4b3755 rba Articles
Links & Downloads

Braz J Anesthesiol

Share this page
Page Sections