The effect of low dose ionizing radiation exposure on dynamic thiol-disulfide homeostasis and ischemia modified albumin levels: an observational study
Efeito da exposição à radiação ionizante de baixa dose na homeostase dinâmica de tiol-dissulfeto e níveis de albumina modificada por isquemia: estudo observacional
Arıcan Sule, Dertli Ramazan, Baktik Suleyman, Hacibeyoglu Gulcin, Erol Atilla, Sinan Oguzhan Ulukaya, Goger Esra, Erel Ozcan
Abstract
Background
The primary objective of this study was to investigate the effect of low dose ionizing radiation exposure on thiol/disulfide homeostasis and ischemia modified albumin levels. The secondary objective is to compare thiol/disulfide homeostasis and ischemia modified albumin levels among the personnel exposed to low dose ionizing radiation in anesthesia application areas, in and out of the operation room.
Methods
The study included a total of 90 volunteers aged between 18 and 65 years old, with 45 personnel working in a setting with potential for radiation exposure (Exposed Group) and 45 personnel in a setting without radiation exposure (Control Group). Their native thiol, total thiol, disulphide, albumine and IMA levels were measured. Exposed group included personnel who were exposed to radiation outside the operating room – Operation room (-) Group and in the operating room – operation room (+) Group.
Results
Albumin, native and total thiol levels were significantly lower in the participants exposed to radiation in the anesthesia application area, no statistically significant difference was found in terms of disulfide and ischemia modified albumin levels. In the operation room (-) group exposed to radiation, native thiol and total thiol values were significantly lower compared to the operation room (+) groups.
Conclusion
Awareness of being in danger of oxidative stress should be established in personnel exposed to radiation in the anesthesia application area following low dose ionizing radiation exposure, and the necessary measures should be taken.
Keywords
Resumo
Justificativa
O objetivo principal do estudo foi investigar o efeito de exposição à radiação ionizante de baixa dose nos níveis de homeostase tiol/dissulfeto e de albumina modificada por isquemia. O objetivo secundário foi comparar os níveis de homeostase tiol/dissulfeto e albumina modificada por isquemia entre indivíduos expostos à radiação ionizante de baixa dose nas áreas de procedimentos anestésicos, dentro e fora da sala de cirurgia.
Método
O estudo incluiu um total de 90 voluntários com idades entre 18 e 65 anos, 45 profissionais que trabalhavam em ambiente de exposição potencial a radiação (Grupo Exposto) e 45 profissionais que trabalhavam em ambiente sem exposição à radiação (Grupo Controle). Foram medidos os níveis de tiol nativo, tiol total, dissulfeto, albumina e albumina modificada por isquemia. O Grupo Exposto era constituído por profissionais expostos a radiação fora da sala de cirurgia – Grupo sala cirúrgica (-) e na sala de cirurgia – Grupo sala de cirurgia (+).
Resultados
Os níveis de albumina, tiol nativo e total foram significantemente mais baixos nos participantes expostos à radiação em área de realização de anestesia, e nenhuma diferença estatisticamente significante foi encontrada para os níveis de dissulfeto e albumina modificada por isquemia. No Grupo exposto sala de cirurgia (-), os valores de tiol nativo e tiol total foram significantemente mais baixos quando comparados ao Grupo sala de cirurgia (+).
Conclusões
Os profissionais expostos à radiação em área de realização de anestesia devem ser conscientizados quanto ao perigo do estresse oxidativo após exposição à radiação ionizante de baixa dose e medidas cabíveis devem ser instituídas.
Palavras-chave
References
1. Ozasa K. Epidemiological research on radiation-induced cancer in atomic bomb survivors. J Radiat Res. 2016;57:112-7.
2. Tubiana M, Feinendegen LE, Yang C, et al. The linear no-threshold relationship is inconsistent with radiation biologic and experimental data. Radiology. 2009;251:13-22.
3. Seong KM, Kwon T, Seo S, et al. Perception of low dose radiation risks among radiation researchers in Korea. PLoS One. 2017;12:e0171777.
4. United Nations Scientific Committee on the Effects of AtomicRadiation [UNSCEAR]. 2000. Biological effects at low radiation doses. United Nations Scientific Committee on the Effects of Atomic Radiation. UNSCEAR Report Vol. II: Effects (Annex G). New York: United Nations. [accessed 2018 Jan 16]. http:// www. unscear.org/docs/publications/2000/UNSCEAR 2000 Report Vol.II.
5. National Research Council 2006 [NRC]. Health Risks from Exposure to Low Levels of Ionizing Radiation: BEIR VII Phase 2. Washington, DC: The National Academies Press. https://doi.org/10.17226/11340.
6. International Commission on Radiological Protection. 2007. The 2007 recommendations of the International Commission on Radiological Protection. ICRP Publication 103. Annals of the ICRP 37. [accessed 2018 Jan 8]. https: // journals. sagepub.com/doi/pdf/10.1177/ ANIB_37_2-4.
7. Banne AF, Amiri A, Pero RW. Reduced level of serum thiols in patients with a diagnosis of active disease. J Anti Aging Med 2003;6:327-34.
8. Erel O, Neselioglu S. A novel and automated assay for thiol/disulphide homeostasis. Clin Biochem 2014;47:326-32.
9. Taverna M, Marie AL, Mira JP, et al. Specific antioxidant properties of human serum albumin. Ann Intensive Care. 2013;3:2-7.
10. Sokolowska M, Krezel A, Dyba M, et al. Short peptides are not reliable models of thermodynamic and kinetic properties of the N-terminal metal binding site in serum albumin. Eur J Biochem. 2002;269:1323-31.
11. Roy D, Quiles J, Gaze D.C. et al. Role of reactive oxygen species on the formation of the novel diagnostic marker ischaemia modified albumin. Heart. 2006;92:113-4.
12. Bar-Or D, Lau E, Winkler JV. A novel assay for cobalt albumin binding and its potential as a marker for myocardial ischemia a preliminary report. J Emerg Med. 2000;19:311-5.
13. Multhoff G, Radons J. Radiation, inflammation, and immune responses in cancer. Front Oncol. 2012;2:58.
14. B. Halliwell, J. Gutteridge Free Radicals in Biology and Medicine Oxford University Press, Oxford, UK (2007).
15. Smith JT, Willey NJ, Hancock JT. Low dose ionizing radiation produces too few reactive oxygen species to directly affect antioxidant concentrations in cells. Biol Lett. 2012;8:594-7.
16. Galván I, Bonisoli Alquati A, Jenkinson S et al. Chronic exposure to low-dose radiation at Chernobyl favours adaptation to oxidative stress in birds. Funct Ecol. 2014;28:1387-403.
17. Pandey B, Kumar A, Tiwari P, et al. Radiobiological basis in management of accidental radiation exposure. Int J Radiat Biol. 2010;86:613-35.
18. Malekirad AA, Ranjbar A, Rahzani K, et al. Oxidative stress in radiology staff. Environ Toxicol Pharmacol. 2005;20:215-8.
19. Eken A, Aydin A, Erdem O, et al. Induced antioxidant activity in hospital staff occupationally exposed to ionizing radiation. Int J Radiat Biol. 2012;88:648-53.
20. Koc U, Tan S, Ertem AG, et al. Evaluation of thiol-disulphide homeostasis in radiation workers. Int J Radiat Biol. 2017;93:705-10.
21. Kundi H, Ates I, Kiziltunc E, et al. A novel oxidative stress marker in acute myocardial infarction; thiol/disulphide homeostasis. Am J Emerg Med. 2015;33:1567-71.
22. Emre S, Demirseren DD, Alisik M, et al. Dynamic thiol/disulfide homeostasis and effects of smoking on homeostasis parameters in patients with psoriasis. Cutan Ocul Toxicol. 2017;36:393-6.
23. Sahin A, Turkoglu S, Tunc N, et al. Is ischemia-modified albumin a reliable tool for the assessment of acute pancreatitis? Ther Clin Risk Manag. 2018;14:627-35.
24. Sinha MK, Vazquez JM, Calvino R, et al. Effects of balloon occlusion during percutaneous coronary intervention on circulating ischemia modified albumin and transmyocardial lactate extraction. Heart 2006;92:1852-3.
25. Gafsou B, Lefevre G, Hennache B, et al. Maternal serum ischemia-modified albumin: a biomarker to distinguish between normal pregnancy and preeclampsia? Hypertens Pregnancy 2010;29:101-11.
26. Michelis R, Kristal B, Snitkovsky T, et al. Oxidative modifications impair albumin quantification. BBRC 2010;401:137-42.
27. Ramos M, Montoro A, Almonacid M, et al. Radiation effects analysis in a group of interventional radiologists using biological and physical dosimetry methods. Eur J Radiol. 2009;75:259-64.
28. Kozanhan B, Inanlı I, Deniz CD, et al. Dynamic thiol disulphide homeostasis in operating theater personel exposed to anestheticgases. Am J Ind Med. 2017;60:1003-9.
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