Subarachnoid meloxicam does not inhibit the mechanical hypernociception on carrageenan test in rats

Lanucha Fidelis da Luz Moura; Silvana Bellini Vidor; Anelise Bonilla Trindade; Priscilla Domingues Mörschbächer; Nilson Oleskovicz; Emerson Antonio Contesini

doi:10.1016/j.bjane.2013.10.020

Scientific Article

Subarachnoid meloxicam does not inhibit the mechanical hypernociception on carrageenan test in rats

Meloxicam subaracnoide não inibe a hipernocicepção mecânica no teste da carragenina em ratos

Lanucha Fidelis da Luz Moura; Silvana Bellini Vidor; Anelise Bonilla Trindade; Priscilla Domingues Mörschbächer; Nilson Oleskovicz; Emerson Antonio Contesini

http://dx.doi.org/10.1016/j.bjane.2013.10.020 Braz J Anesthesiol, vol.65, n2, p.124-129, 2015

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Abstract

BACKGROUND AND OBJECTIVE: Evaluate the antinociceptive effects of subarachnoid meloxicam on the mechanical hypernociception induced by carrageenan in rats. METHODS: Randomized controlled trial. Eighteen adult male Wistar rats underwent a cannula implantation into the subarachnoid space and were randomly divided into two groups: Group I received saline solution 5 µL, while Group II received meloxicam 30 mg. The mechanical hypernociception was induced by intraplantar injection of carrageenan and evaluated using a digital analgesy meter every 30 min during a 4-h period. The results were recorded as the Δ withdrawal threshold (in g), calculated by subtracting the measurement value after treatment from baseline. RESULTS: The Δ withdrawal threshold mean values were lower in the group of patients treated with meloxicam over all time points between 45 and 165 min, however, there was no statistical significance (p = 0.835) for this difference. CONCLUSION: Subarachnoid meloxicam at a dose of 30 µg animal-1 did not suppress the mechanical hypernociception in a model of inflammatory pain induced by intraplantar administration of carrageenan in rats. The data suggest that other dosages should be investigated the drug effect is discarded.

Keywords

NSAIDs, Carrageenan, Pain, Spinal cord

Resumo

JUSTIFICATIVA E OBJETIVO: Avaliar os efeitos antinociceptivos do meloxicam subaracnóideo sobre a hipernocicepção mecânica induzida pela carragenina em ratos. MÉTODOS: Estudo randômico e controlado. Dezoito ratos Wistar, machos adultos, foram submetidos à implantação de uma cânula subaracnóidea, e aleatoriamente distribuídos em dois grupos: o Grupo I (GI) recebeu 5 µL de solução salina, enquanto que ao Grupo II (GII) foram administrados 30 µg de meloxicam, ambos pela via subaracnóidea. A hipernocicepção mecânica foi induzida pela injeção intraplantar de carragenina e avaliada com o emprego de um analgesímetro digital a cada 30 minutos durante um período de 4 horas. Os resultados foram registrados como o Δ do limiar de retirada (g), calculado subtraindo-se o valor das mensurações após os tratamentos, do valor basal. RESULTADOS: Os valores médios do Δ do limiar de retirada foram menores no grupo tratado com meloxicam ao longo de todos os momentos de avaliação entre 45 e 165 minutos, contudo não foi demonstrada significância estatística (p = 0,835) para essa diferença. CONCLUSÃO: A administração subaracnóidea do meloxicam na dose de 30 µg.animal-1 não foi capaz de suprimir a hipernocicepção mecânica em um modelo de dor inflamatória induzida pela administração intraplantar de carragenina em ratos. Os dados sugerem que outras doses sejam pesquisadas antes que o efeito do fármaco seja descartado.

Palavras-chave

AINE, Carragenina, Dor, Medula espinhal

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Subarachnoid meloxicam does not inhibit the mechanical hypernociception on carrageenan test in rats

Meloxicam subaracnoide não inibe a hipernocicepção mecânica no teste da carragenina em ratos

Lanucha Fidelis da Luz Moura; Silvana Bellini Vidor; Anelise Bonilla Trindade; Priscilla Domingues Mörschbächer; Nilson Oleskovicz; Emerson Antonio Contesini

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