Brazilian Journal of Anesthesiology
https://bjan-sba.org/article/doi/10.1016/j.bjan.2013.02.001
Brazilian Journal of Anesthesiology
Scientific Article

Avaliação in vitro das propriedades antimicrobianas de vasopressores

In vitro evaluation of antimicrobial features of vasopressors

Habib Bostan; Yakup Tomak; Sengul Alpay Karaoglu; Basar Erdivanli; Volkan Hanci

Downloads: 0
Views: 695

Resumo

Justificativa e objetivo: os medicamentos administrados como perfusão intravenosa podem ser contaminados durante as várias etapas de produção ou preparação. No entanto, estudos sobre os efeitos antibacterianos de vasopressores são muito raros. Este estudo investiga a atividade antimicrobiana in vitro das formas de vasopressores usados clinicamente. Materiais e métodos: atividades antimicrobianas in vitro de substâncias vasopressoras de diferentes concentrações foram investigadas com o uso da técnica de microdiluição. Os microrganismos empregados no teste foram: Escherichia coli ATCC 25922, Yersinia pseudotuberculosis ATCC 911, Pseudomonas aeruginosa ATCC 10145, Listeria monocytogenes ATCC 43251, Enterococcus faecalis ATCC 29212, Staphylococcus aureus ATCC 25923, Bacillus cereus 702 Roma, Mycobacterium smegmatis ATCC607, Candida albicans ATCC 60193 e Saccharomyces cerevisiae RSKK 251. Os ensaios antibacterianos foram feitos em caldo de cultura Mueller-Hinton (pH 7,3) e os ensaios antifúngicos em solução tampão de base nitrogenada para levedura (pH 7,0). Resultados: duas preparações diferentes de dopamina mostraram atividade antimicrobiana. Nenhuma outra substância do estudo mostrou qualquer atividade antimicrobiana. Conclusões: em nossa opinião, os efeitos antibacterianos da dopamina podem ser vantajosos para inibir a propagação de contaminação bacteriana durante a preparação das soluções para perfusão. Contudo, salientamos a importância do seguimento rigoroso das diretrizes de esterilização dos equipamentos e de assepsia durante todos os procedimentos feitos em unidades de terapia intensiva.

Palavras-chave

Atividade antimicrobiana, Vasopressores, Contaminacão de medicamentos

Abstract

Background: Drugs administered as intravenous infusion may be contaminated during several stages of production or preparation. However studies focusing on antibacterial effects of vasopressor drugs are very rare. This study investigates the in vitro antimicrobial activity of the clinically used forms of vasopressors. Materials and methods: In vitro antimicrobial activities of vasopressor drugs of different concentrations were investigated by using the micro dilution technique. Microorganisms used in the test were Escherichia coli ATCC 25922, Yersinia pseudotuberculosis ATCC 911, Pseudomonas aeruginosa ATCC 10145, Listeria monocytogenes ATCC 43251, Enterococcus faecalis ATCC 29212, Staphylococcus aureus ATCC 25923, Bacillus cereus 702 Roma, Mycobacterium smegmatis ATCC607, Candida albicans ATCC 60193, and Saccharomyces cerevisiae RSKK 251. Antibacterial assays were performed in Mueller-Hinton broth at pH 7.3 and antifungal assays were performed in buffered Yeast Nitrogen Base at pH 7.0. Results: Two different dopamine preparations showed antimicrobial activity. No other study drug showed any antimicrobial activity. Conclusions: In our opinion, dopamine's antibacterial effects may be advantageous for inhibiting the spread of bacterial contamination during the preparation of the infusion solutions. However, it is important that strict guidelines regarding the need for sterile equipment and deliverables be adhered to during all procedures performed in the intensive care units.

Keywords

Antimicrobial activities, Vasopressor drugs, Drug contamination

References

Levy B, Collin S, Sennoun N. Vascular hyporesponsiveness to vasopressors in septic shock: from bench to bedside. Intensive Care Med. ;36:2019-2029.

Póvoa P, Carneiro AH. Adrenergic support in septic shock: a critical review. Hosp Pract (Minneap). ;38:62-73.

Lyte M. The role of catecholamines in Gram-negative sepsis. Med Hypotheses. ;37:258-255.

Lyte M, Ernst S. Catecholamine induced growth of Gram negative bacteria. Life Sci. ;50:203-212.

Neal CP, Freestone PP, Maggs AF, Haigh RD, Williams PH, Lyte M. Catecholamine inotropes as growth factors for Staphylococcus epidermidis and other coagulase-negative staphylococci. FEMS Microbiol Lett. ;194:169-163.

Methods for determining bactericidal activity of antimicrobial agents; approved guideline. .

Woods GL, Brown-Elliott BA, Desmond EP. Susceptibility testing of mycobacteria, nocardiae, and other aerobic actinomycetes; approved standard. ;23.

Hanci V, Cömert F, Ayoglu H, Kulah C, Yurtlu S, Turan IO. Evaluation of the antimicrobial effects of atracurium, rocuronium and mivacurium. Antimicrobial effects of muscle relaxants. Drugs Ther Stud. ;1:e2.

Ayoglu H, Kulah C, Turan I. Antimicrobial effects of two anaesthetic agents: dexmedetomidine and midazolam. Anaesth Intensive Care. ;36:684-681.

Graystone S, Wells MF, Farrell DJ. Do intensive care drug infusions support microbial growth. Anaesth Intensive Care. ;25:640-642.

Crowther J, Hrazdil J, Jolly DT, Galbraith JC, Greacen M, Grace M. Growth of microorganisms in propofol, thiopental, and a 1:1 mixture of propofol and thiopental. Anesth Analg. ;82:478-475.

Sosis MB, Braverman B, Villaflor E. Propofol, but not thiopental, supports the growth of Candida albicans. Anesth Analg. ;81:132-134.

Keleș GT, Kurutepe S, Tok D. Comparison of antimicrobial effects of dexmedetomidine and etomidate-lipuro with those of propofol and midazolam. Eur J Anaesthesiol. ;23:1040-1037.

Sandrini SM, Shergill R, Woodward J. Elucidation of the mechanism by which catecholamine stress hormones liberate iron from the innate immune defense proteins transferrin and lactoferrin. J Bacteriol. ;192:587-594.

Freestone PPE, Haigh RD, Lyte M. Blockade of catecholamine-induced growth by adrenergic and dopaminergic receptor antagonists in Escherichia coli O157:H7, Salmonella enterica and Yersinia enterocolitica. Microbiol. ;7:8.

Ercan S, Oztürk N, Celik-Ozenci C, Gungor NE, Yargicoglu P. Sodium metabisulfite induces lipid peroxidation and apoptosis in rat gastric tissue. Toxicol Ind Health. ;26:425-431.

Baker MT, Dehring DJ, Gregerson MS. Sulfite supported lipid peroxidation in propofol emulsions. Anesthesiology. ;97:1167-1162.

Olofsson AC, Hermansson M, Elwing H. N-acetyl-L-cysteine affects growth, extracellular polysaccharide production, and bacterial biofilm formation on solid surfaces. Appl Environ Microbiol. ;69:4822-4814.

Mansouri MD, Darouiche RO. In vitro antimicrobial activity of N-acetylcysteine against bacteria colonising central venous catheters. Int J Antimicrob Agents. ;29:471-483.

Huynh HQ, Couper RT, Tran CD, Moore L, Kelso R, Butler RN. N-acetylcysteine, a nove treatment for Helicobacter pylori infection. Dig Dis Sci. ;49:1861-1853.

Aslam S, Trautner BW, Ramanathan V, Darouiche RO. Combination of tigecycline and N-acetylcysteine reduces biofilm-embedde bacteria on vascular catheters. Antimicrob Agents Chemother. ;51:1558-1556.

5dcdb3850e8825435cbf58f1 rba Articles
Links & Downloads

Braz J Anesthesiol

Share this page
Page Sections